Healing Effect of Ziziphus Honey on Extracted Tooth Socket in Humans by Evaluating the Levels of BMP-2 and OPN


Ziziphus honey can improve bone healing at extracted tooth sockets in humans by increasing salivary levels of bone markers, OPN and BMP-2.

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Parallel Assignment
    • Primary Purpose: Supportive Care
    • Masking: Single (Participant)
  • Study Primary Completion Date: March 16, 2020

Detailed Description

objectives of study: To study the effects of ziziphus honey on bone healing of extracted tooth socket in humans by comparing 1) Levels of BMP-2 and OPN in experimental and control groups at different intervals and 2) By radiographically measuring RBD of extraction sockets in experimental and control groups. Aim of study: Post extraction ridge resorption is a major concern for future tooth prosthesis or implant placement. Honey can possibly help to improve bone healing and minimize ridge resorption after extractions. Materials and Methods: Randomized controlled trial with simple random sampling of healthy individuals will be done. Total number of 30 participants will be equally divided into experimental group and control group. After tooth extractions in both groups, ziziphus honey will be injected into extracted sockets of experimental group while control group will be left as it is. Levels of BMP-2 and OPN will be tested in saliva through ELISA technique at day 0, 3 and 7 of tooth extraction and RBD will be measured on periapical radiographs at day 3, 21 and 40 in both groups. Keywords: Bone morphogenetic protein-2 (BMP-2), Osteopontin (OPN), Relative bone density (RBD), Enzyme linked immunosorbent assay (ELISA).


  • Drug: ziziphus honey
    • ziziphus honey obtained form ziziphus spina-christi trees also called sidr tree

Arms, Groups and Cohorts

  • No Intervention: control group
    • only atraumatic extractions were done, honey was not applied
  • Experimental: experimental group
    • ziziphus honey was applied into the sockets after tooth extractions in experimental group

Clinical Trial Outcome Measures

Primary Measures

  • levels of BMP-2
    • Time Frame: 1 week
    • bone morphogenetic protein-2 levels to access amount of bone healing
  • levels of OPN
    • Time Frame: 1 week
    • Osteopontin levels to access amount of bone healing
  • Relative bone density
    • Time Frame: 40 days
    • bone quality confirmed on periapical radiographs in both groups

Participating in This Clinical Trial

Inclusion Criteria

1. Healthy patients of either gender with adult age 2. No known hypersensitivity to honey. 3. Permanent maxillary or mandibular molars (first or second molars) Exclusion Criteria:

1. History of radiotherapy or chemotherapy 2. Diabetic and hypertensive patients. 3. Pregnant and lactating females. 4. Smoking or consumption of alcohol 5. Bone conditions like osteoporosis etc. 6. Patients on oral contraceptives or steroid therapy 7. Subjects taking antibiotics or NSAIDS within one week.

Gender Eligibility: All

Minimum Age: 18 Years

Maximum Age: 40 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • Postgraduate Medical Institute, Lahore
  • Provider of Information About this Clinical Study
    • Principal Investigator: Momina khalid, Principal investigator – Postgraduate Medical Institute, Lahore
  • Overall Official(s)
    • Momina Khalid, BDS, Principal Investigator, PGMI, Lahore


Mendes RM, Silva GA, Lima MF, Calliari MV, Almeida AP, Alves JB, Ferreira AJ. Sodium hyaluronate accelerates the healing process in tooth sockets of rats. Arch Oral Biol. 2008 Dec;53(12):1155-62. doi: 10.1016/j.archoralbio.2008.07.001. Epub 2008 Aug 9.

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