Muscle Response to High Intensity Interval Resistance Training (HIIRT)

Overview

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Full Title of Study: “Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training”

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Crossover Assignment
    • Primary Purpose: Basic Science
    • Masking: Single (Outcomes Assessor)
  • Study Primary Completion Date: May 15, 2012

Detailed Description

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Interventions

  • Other: High Intensity Interval Resistance Training
    • Subjects trained one leg with HIIRT
  • Other: Traditional Resistance Training
    • Subjects trained the other leg with HIIRT

Arms, Groups and Cohorts

  • Experimental: High Intensity Interval Resistance Training (HIIRT)
    • HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2’30″ rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.
  • Active Comparator: Traditional Resistance Training (TRT)
    • TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75″ of rest between sets.

Clinical Trial Outcome Measures

Primary Measures

  • pAMPKThr172 in skeletal muscle tissue measured by immunoblot
    • Time Frame: 24 hours
    • measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy
  • pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot
    • Time Frame: 24 hours
    • measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy
  • pS6Ser235/236 in skeletal muscle tissue measured by immunoblot
    • Time Frame: 24 hours
    • measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy
  • p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot
    • Time Frame: 24 hours
    • measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy
  • ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot
    • Time Frame: 24 hours
    • ACC phosphorylation in skeletal muscle tissue taken by biopsy
  • IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • IGF-1 mRNA in skeletal muscle tissue taken by biopsy
  • IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hoursn
    • IGF-1ea mRNA in skeletal muscle tissue taken by biopsy
  • MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • MGF mRNA in skeletal muscle tissue taken by biopsy
  • STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hoursn
    • STARS mRNA in skeletal muscle tissue taken by biopsy
  • Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • Myogenin mRNA in skeletal muscle tissue taken by biopsy
  • IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • IL-6 mRNA in skeletal muscle tissue taken by biopsy
  • PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • PGC1a4 mRNA in skeletal muscle tissue taken by biopsy
  • PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • PGC1a mRNA in skeletal muscle tissue taken by biopsy
  • Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • Atrogin mRNA in skeletal muscle tissue taken by biopsy
  • Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • Beclin mRNA in skeletal muscle tissue taken by biopsy
  • Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
    • Time Frame: 24 hours
    • Myostatin mRNA in skeletal muscle tissue taken by biopsy

Participating in This Clinical Trial

Inclusion Criteria

  • moderate active
  • experience with resistance training

Exclusion Criteria

  • history of recent myocardial infarction,
  • severe cardiac arrhythmia,
  • unstable angina,
  • poorly controlled hypertension,
  • poorly controlled diabetes mellitus,
  • frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Gender Eligibility: All

Minimum Age: 20 Years

Maximum Age: 30 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • University of Padova
  • Collaborator
    • University of Roma La Sapienza
  • Provider of Information About this Clinical Study
    • Principal Investigator: Antonio Paoli, Full Professor – University of Padova
  • Overall Official(s)
    • Antonio Paoli, MD, Study Director, University of Padova

References

Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011.

Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.

Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.

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