Shake It Up: Lipidomics of Lipoproteins and Diet

Overview

The purpose of this research is to understand how dietary fat is related to cardiovascular disease (CVD). This study tests the effect of different dietary fats on the level of fat in the blood and the particles that transport fat in the blood. The researchers want to know if these particles change after eating meals made with different fats.

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Crossover Assignment
    • Primary Purpose: Basic Science
    • Masking: None (Open Label)
  • Study Primary Completion Date: December 2024

Detailed Description

The overall objective of this work is to discover how the lipidomic composition of postprandial lipoproteins responds to dietary fats of differing fatty acid composition by comparing meals rich in saturated fatty acid (SFA) (from dairy cream) versus polyunsaturated fatty acids (PUFA) (from soybean oil). The information derived from these studies will provide mechanistic insight into postprandial lipid metabolism and will support efforts to identify optimal dietary oil intakes for people.

Interventions

  • Other: Heavy Cream
    • Participants will receive a meal in the form of a fruit-containing shake containing 30 g of fat prepared with heavy cream.
  • Other: Soybean Oil
    • Participants will receive a meal in the form of a fruit-containing shake containing 30 g of fat prepared with soybean oil.

Arms, Groups and Cohorts

  • Experimental: Heavy Cream
    • Participants will receive a meal containing 30 g of fat prepared with heavy cream.
  • Experimental: Soybean Oil
    • Participants will receive a meal containing 30 g of fat prepared with soybean oil.

Clinical Trial Outcome Measures

Primary Measures

  • Change in lipidomic composition of plasma post-prandial lipoproteins as assessed by incremental area under the curve (iAUC)
    • Time Frame: 0, 1, 2, 4, 6, 8 hours post meal intake

Participating in This Clinical Trial

Inclusion Criteria

  • Normal to overweight (BMI 18.0-29.9 kg/m^2
  • Willing to comply with the demands of the experimental protocol

Exclusion Criteria

  • Inability to provide consent
  • Have fasting blood glucose <60 mg/dL or >126 mg/dL
  • Have fasting blood cholesterol >300 mg/dL
  • Have fasting blood triglyceride <100 mg/dL or >300 mg/dL
  • Have uncontrolled hypertension (blood pressure >140/90 mm Hg)
  • Take prescription medications for blood sugar or lipid management or anti-inflammatory steroids (e.g. cortisone)
  • Over the counter use of supplements (fish oil, flax, plant sterol or sterol esters) and unwilling to discontinue use for 6 weeks prior to study initiation
  • Have a history of an eating disorder, unusual dietary pattern (extreme nutrient or food group restriction; erratic meals due to shift work, etc.)
  • Have established cardiovascular, pulmonary, and/or a metabolic disease such as diabetes
  • Have cancer
  • Use tobacco products or nicotine in any form including snuff, pills, and patches, or e-cigarettes/vaping in the past 6 weeks
  • Have alcohol, anabolic steroids, or other substance abuse issues
  • Consume more than 3 alcoholic drinks/week
  • Are lactose intolerant or have an allergy to dairy foods

Gender Eligibility: Male

Minimum Age: 18 Years

Maximum Age: 60 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • USDA Grand Forks Human Nutrition Research Center
  • Provider of Information About this Clinical Study
    • Principal Investigator: Matthew Picklo, Supervisory Research Physiologist – USDA Grand Forks Human Nutrition Research Center
  • Overall Official(s)
    • Matthew Picklo, PhD, Principal Investigator, USDA Grand Forks Human Nutrition Research Center
  • Overall Contact(s)
    • Matthew Picklo, PhD, 701-795-8380, matthew.picklo@usda.gov

Clinical trials entries are delivered from the US National Institutes of Health and are not reviewed separately by this site. Please see the identifier information above for retrieving further details from the government database.

At TrialBulletin.com, we keep tabs on over 200,000 clinical trials in the US and abroad, using medical data supplied directly by the US National Institutes of Health. Please see the About and Contact page for details.