Serum Folic Acid Status, Genotyping MTHFR 677C–>T, Plasma Homocysteine, DNA Methylation of IGF2 Gene and Microbiota of the Children: Mother-Children Cohort Study

Overview

This study is part of the East Jakarta Cohort Study to assess the current micronutrient status of the mothers and their children. The study also includes the assessment of genotyping of MTHFR 677C–>T of the mother and DNA methylation of IGF2 gene of the children.

Full Title of Study: “Serum Folic Acid Status, Genotyping MTHFR 677C–>T of the Mother and Serum Folic Acid Status, DNA Methylation of IGF2 Gene of the Children: East Jakarta Cohort Study”

Study Type

  • Study Type: Observational
  • Study Design
    • Time Perspective: Prospective
  • Study Primary Completion Date: November 20, 2018

Detailed Description

The initial study recruited 315 mothers in the public health centre and one district hospital in East Jakarta. The follow-up study is able to re-visit the 127 of them. This also includes 127 peers of the subject that lives in the same neighbourhood. The design of the study is case-cohort design, part study of the existing cohort. The assessment of the follow-up study includes the mother and the children born in the initial study. Anthropometric measurement, socioeconomic data from a structured questionnaire, biomarker assessment, blood pressure were obtained from the subjects.

Arms, Groups and Cohorts

  • East Jakarta Cohort Study
    • One group included in the study was originated from mothers who participated in the first data collection pregnancy. The follow-up study included mothers and their children born as the subject in the follow-up in the study. This study also includes peer of the subjects that lived in the same neighbourhood since the first cohort study and have children in the same age with cohort participant

Clinical Trial Outcome Measures

Primary Measures

  • Serum folic acid status
    • Time Frame: December 2018 – March 2019
    • The measurement of serum folic acid status is using LC-MS/MS
  • Genotyping MTHFR 677C–>T
    • Time Frame: December 2018 – March 2019
    • The assessment of MTHFR 677C–>T using Real Time PCR
  • DNA methylation of IGF2 gene
    • Time Frame: December 2018 – March 2019
    • DNA methylation of IGF2 gene using pyrosequencing method

Secondary Measures

  • Serum Homocysteine
    • Time Frame: December 2018-March 2019
    • The measurement of serum homocysteine using Electrochemiluminescen
  • Serum Adiponectin
    • Time Frame: December 2018-March 2019
    • The measurement of serum adiponection using electrochemiluminescence
  • Blood pressure
    • Time Frame: December 2018-March 2019
    • The blood pressure measurement using semi automatic device (Omron HBP1300)

Participating in This Clinical Trial

Inclusion Criteria

  • participant in the previous study – apparently healthy – data complete – voluntarily to participate Exclusion Criteria:

  • not complete data – migrate – refuse to participate in the study

Gender Eligibility: All

Minimum Age: 3 Years

Maximum Age: 40 Years

Are Healthy Volunteers Accepted: No

Investigator Details

  • Lead Sponsor
    • Indonesia University
  • Collaborator
    • Ministry of Research and Higher Education of Republic Indonesia
  • Provider of Information About this Clinical Study
    • Principal Investigator: Lestari Octavia, MSc – Indonesia University
  • Overall Official(s)
    • Rina RA Agustina, Doctoral, Study Director, Indonesia University

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