Effect of Mango on Gut Microbiota and Metabolic Health

Overview

Mango is rich in bioactive compounds such as dietary polyphenols and carotenoids, which may explain its beneficial effect on health. Polyphenols accumulate in the gut where they can positively modulate the microbiota. As gut microbiota may have a strong influence on cardiometabolic health, we hypothesize that mango consumption improves metabolic profile in overweight or obese individuals through beneficial changes in gut microbiota. The study of metagenomics, transcriptomics and metabolomics will be used to validate this hypothesis.

Full Title of Study: “Metabolic Adaptations and Role of Nutritionally-Mediated Gut Microbiota Changes Following Mango Consumption in Overweight Men and Women : The MANGO Pilot Study”

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: N/A
    • Intervention Model: Single Group Assignment
    • Primary Purpose: Prevention
    • Masking: None (Open Label)
  • Study Primary Completion Date: December 20, 2019

Interventions

  • Dietary Supplement: Mango consumption
    • Daily consumption of 280 g of frozen mangos during 8 weeks. The daily dose in equivalent to a 2-cups serving.

Arms, Groups and Cohorts

  • Experimental: Mango consumption

Clinical Trial Outcome Measures

Primary Measures

  • Changes in plasma insulin concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma glucose concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma triglycerides concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma total cholesterol concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma HDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma LDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks

Secondary Measures

  • Changes in waist circumference of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in hip circumference of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in waist to hip circumference ratio of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
    • Waist and hip circumference will be combined to report waist to hip ratio
  • Height of overweight/obese men and women taking frozen mangos daily
    • Time Frame: Baseline
  • Changes in weight of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in body mass index (BMI) of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
    • Weight and height will be combined to report BMI
  • Changes in systolic blood pressure of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in diastolic blood pressure of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in insulin secretion of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in insulin sensitivity of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in glycated hemoglobin (HbA1c) in overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma tumor necrosis factor (TNF-alpha) concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma interleukin-6 (IL-6) concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma high-sensitivity C-reactive protein (hs-CRP) concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in gene expression in peripheral blood mononuclear cells (PBMC) of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
    • Gene-level expression from >20,000 annotated genes will be examined
  • Changes in plasma metabolites concentration in overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
    • Metabolites include acylcarnitines, glycerophospholipids, sphingolipids, sugars, amino acids, biogenic amines
  • Changes in gut microbiota composition in overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
    • Stool samples will be collected and whole metagenome shotgun sequencing analysis will be conducted
  • Changes in plasma lipopolysaccharide (LPS) concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks
  • Changes in plasma LPS binding protein (LBP) concentration of overweight/obese men and women taking frozen mangos daily
    • Time Frame: 8 weeks

Participating in This Clinical Trial

Inclusion Criteria

  • Healthy men and premenopausal women – At least one of the following : BMI between 25 and 40 kg/m2 or Waist circumference ≥ 80 cm for women and ≥ 94 cm for men – At least one of the following : TG ≥ 1.35 mmol/L or fasting insulinemia ≥ 42 pmol/L – Caucasian origin Exclusion Criteria:

  • Nicotine use – Metabolic disorders or use of medication that may affect parameters measured in the study – Use of dietary supplements or natural health product that may affect parameters measured in the study – Use of antibiotics in the last 3 months – Surgery in the last 3 months or planned during the study timeline – Alcohol consumption >2 drinks/day – Weight change >5% in the last 3 months – Mango aversion, allergy or intolerance – Dietary restrictions (vegetarian, gluten avoidance…) – Berries consumption >1 serving/day – Pregnancy, breastfeeding or pregnancy planned within 3-6 months

Gender Eligibility: All

Minimum Age: 18 Years

Maximum Age: 55 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • Laval University
  • Provider of Information About this Clinical Study
    • Principal Investigator: Charles Couillard, Professor – Laval University
  • Overall Official(s)
    • Charles.Couillard@fsaa.ulaval.ca Couillard, PhD, Principal Investigator, Laval University

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