ECigs, Inflammation and Oxidative Stress

Overview

Randomized controlled trial of acute use of electronic cigarette effect on oxidative stress and inflammation.

Full Title of Study: “E-Cigarettes and Monocytes”

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Crossover Assignment
    • Primary Purpose: Basic Science
    • Masking: Single (Outcomes Assessor)
  • Study Primary Completion Date: February 8, 2023

Detailed Description

Participants will have an acute e-cig exposure on 2 occasions 1) e-cigarette 2) sham e-cig (empty e-cig) control. Blood will be collected before and after E-cig use and analyzed for biomarkers of inflammation and oxidative stress.

Interventions

  • Other: e-cigarette
    • use e-cig for 30 minutes
  • Other: empty (-cigarette sham control)
    • use empty e-cig (sham control)

Arms, Groups and Cohorts

  • Experimental: E-cig
    • One time exposure to e-cig
  • Experimental: sham
    • One time exposure to empty e-cig

Clinical Trial Outcome Measures

Primary Measures

  • Cellular Reactive Oxygen Species, units = % fluorescence
    • Time Frame: within 4 hours of E-cig use
    • cellular oxidative stress, including cytoplasmic and mitochondrial oxidative stress, in viable cells

Secondary Measures

  • CD 14+ Monocyte, units = % of total cells
    • Time Frame: within 4 hours of e-cig use
    • CD 14+ Monocyte, % of total cells will be measured with antibodies and stable dye

Participating in This Clinical Trial

Inclusion Criteria

  • Healthy Exclusion Criteria:

  • Cardiac disease Respiratory disease Diabetes

Gender Eligibility: All

Minimum Age: 21 Years

Maximum Age: 45 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • University of California, Los Angeles
  • Collaborator
    • Tobacco Related Disease Research Program
  • Provider of Information About this Clinical Study
    • Principal Investigator: Holly R Middlekauff, Principal Investigator – University of California, Los Angeles

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