ECigs, Inflammation and Oxidative Stress
Overview
Randomized controlled trial of acute use of electronic cigarette effect on oxidative stress and inflammation.
Full Title of Study: “E-Cigarettes and Monocytes”
Study Type
- Study Type: Interventional
- Study Design
- Allocation: Randomized
- Intervention Model: Crossover Assignment
- Primary Purpose: Basic Science
- Masking: Single (Outcomes Assessor)
- Study Primary Completion Date: February 8, 2023
Detailed Description
Participants will have an acute e-cig exposure on 2 occasions 1) e-cigarette 2) sham e-cig (empty e-cig) control. Blood will be collected before and after E-cig use and analyzed for biomarkers of inflammation and oxidative stress.
Interventions
- Other: e-cigarette
- use e-cig for 30 minutes
- Other: empty (-cigarette sham control)
- use empty e-cig (sham control)
Arms, Groups and Cohorts
- Experimental: E-cig
- One time exposure to e-cig
- Experimental: sham
- One time exposure to empty e-cig
Clinical Trial Outcome Measures
Primary Measures
- Cellular Reactive Oxygen Species, units = % fluorescence
- Time Frame: within 4 hours of E-cig use
- cellular oxidative stress, including cytoplasmic and mitochondrial oxidative stress, in viable cells
Secondary Measures
- CD 14+ Monocyte, units = % of total cells
- Time Frame: within 4 hours of e-cig use
- CD 14+ Monocyte, % of total cells will be measured with antibodies and stable dye
Participating in This Clinical Trial
Inclusion Criteria
- Healthy Exclusion Criteria:
- Cardiac disease Respiratory disease Diabetes
Gender Eligibility: All
Minimum Age: 21 Years
Maximum Age: 45 Years
Are Healthy Volunteers Accepted: Accepts Healthy Volunteers
Investigator Details
- Lead Sponsor
- University of California, Los Angeles
- Collaborator
- Tobacco Related Disease Research Program
- Provider of Information About this Clinical Study
- Principal Investigator: Holly R Middlekauff, Principal Investigator – University of California, Los Angeles
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