Mitochondrial Function in Circulating Cells and Muscle Tissue

Overview

The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.

Study Type

  • Study Type: Observational
  • Study Design
    • Time Perspective: Cross-Sectional
  • Study Primary Completion Date: April 26, 2018

Detailed Description

Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.

Arms, Groups and Cohorts

  • Healthy lean
    • Healthy lean women, 25-35 years
  • Overweight/obese with/without IR
    • Overweight/obese with/without insulin resistant women, 25-35 years

Clinical Trial Outcome Measures

Primary Measures

  • Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women
    • Time Frame: During the visit, approximately 0.5 days
    • Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry. From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects.

Participating in This Clinical Trial

Inclusion Criteria

  • Women – 25-35 years of age – Either healthy lean (BMI<25) or insulin resistant and obese (BMI >30) Exclusion Criteria:

  • Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication – Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate <45), or heart problems

Gender Eligibility: Female

Minimum Age: 25 Years

Maximum Age: 35 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • University of Arkansas
  • Collaborator
    • Arkansas Children’s Hospital Research Institute
  • Provider of Information About this Clinical Study
    • Sponsor
  • Overall Official(s)
    • Elisabet Borsheim, PhD, Principal Investigator, Arkansas Children’s Nutrition Center

Citations Reporting on Results

Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Borsheim E. A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab. 2019 Sep 1;317(3):E503-E512. doi: 10.1152/ajpendo.00084.2019. Epub 2019 Jun 18.

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