Changes in Alveolar Macrophage Function During Acute Lung Injury

Overview

In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Study Type

  • Study Type: Observational
  • Study Design
    • Time Perspective: Prospective
  • Study Primary Completion Date: December 2017

Detailed Description

Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Interventions

  • Procedure: Bronchoscopy
    • Bronchoscopy will be implemented under sedation in both groups.

Arms, Groups and Cohorts

  • acute lung injury
    • Patients with a diagnosis of acute lung injury (ALI) under mechanical ventilation.Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.
  • control
    • Patient under mechanical ventilation without any lung diseases. Patients will underwent bronchoscopy for bronchoalveolar lavage fluid.

Clinical Trial Outcome Measures

Primary Measures

  • alveolar macrophages (AMs) phagocytic index
    • Time Frame: 7 days after admission
    • AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.

Secondary Measures

  • 30 days mortality
    • Time Frame: 30 days after admission

Participating in This Clinical Trial

Inclusion Criteria

  • Patients in ICU with diagnosed ALI under mechanical ventilation Exclusion Criteria:

  • Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis

Gender Eligibility: All

Minimum Age: 18 Years

Maximum Age: N/A

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • West China Hospital
  • Provider of Information About this Clinical Study
    • Principal Investigator: Chunling Jiang, Dr. – West China Hospital
  • Overall Official(s)
    • Jin Liu, Study Chair, West China Hospital
  • Overall Contact(s)
    • Chunling Jiang, jiang_chunling@yahoo.com

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