Study of the Efficiency of Hydroxychloroquine on the Endothelial Dysfunction and Its Vascular Consequences During the Antiphospholipid Syndrome

Overview

This study evaluates the benefits of hydroxychloroquine on arterial function in antiphospholipid syndrome. Briefly, the patients will be randomized in two groups, one will receive hydroxychloroquine and standard treatment, the other will receive placebo in addition of standard treatment.

Full Title of Study: “Efficiency of Hydroxychloroquine on the Endothelial Dysfunction in Antiphospholipid Syndrome (APLAQUINE)”

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Parallel Assignment
    • Primary Purpose: Treatment
    • Masking: Single (Outcomes Assessor)
  • Study Primary Completion Date: December 2018

Detailed Description

Antiphospholipid syndrome (APS) is an autoimmune disease characterized by recurrent thrombotic events and miscarriages, with persistently positive antiphospholipid antibodies (aPL). APS may be isolated (primary APS) or associated to a connective tissue disease, most often systemic lupus erythematous (SLE). Pathogenic effects of aPL were first described by the demonstration that in vitro incubation of endothelial cells or monocytes with aPL induce an endothelial dysfunction characterized by pro-coagulant (overexpression of tissue factor and modulation of protein C and S), pro-inflammatory (increased level of IL-6(interleukin 6) , IL-1β and TNFα) and pro-adhesive (increased levels of ICAM-1(intercellular adhesion molecule ), VCAM-1 (vascular endothelial cell adhesion molecule) and E-selectin) phenotypes. In parallel the investigators and others reported that endothelial function, assessed by flow mediated dilatation, is altered in patients with primary and secondary forms of APS. Although a role for TLR (toll-like receptor )-mediated NFkB translocation has been advanced, the pathogenic mechanisms that lead to in vivo endothelial injury in APS are incompletely understood. In an experimental model, the investigators demonstrated that passive transfer of human aPL to mice induced a marked endothelial dysfunction assessed ex vivo in small resistance arteries, and an increase in TNFα levels. Moreover, the investigators group have demonstrated that patients with primary arterial APS display endothelial dysfunction and structural arterial changes, associated with a pro-oxidative and pro-coagulant state and with activation of the TLR2 and TLR4 signalling pathways. Recently, in a preliminary study the investigators have found that endothelial glycocalyx which is an important part of the vascular barrier and which is intimately linked to the homeostatic functions of the endothelium was altered in APL patients. Hydroxychloroquine (HCQ) is an antimalarial drug, also used to treat rheumatic diseases such as SLE. There is experimental evidence to suggest a direct role of hydroxychloroquine on the pathophysiology of APS: it directly reduces the binding of antibodies on the phospholipid bilayers, protects the annexin A5 anticoagulant shield and it reverses platelet adhesion induced by aPL. Furthermore it is known to decrease the expression of lysosomal TLRs, but also extra lysosomal TLR2 and TLR4. The aim of this study is to investigate whether treatment with hydroxychloroquine modulates vascular endothelial function in patients.

Interventions

  • Drug: Hydroxychloroquine
    • endothelial function is assessed by measuring the flow mediated dilatation of humeral artery in response to ischemia. the dilatation is evaluated by echotracking. glycocalyx thickness is measured by the study of sublingual microcirculation with SDF imaging. oxydative, inflammatory and coagulation parameters is assessed on plasma samples.
  • Drug: placebo
    • endothelial function is assessed by measuring the flow mediated dilatation of humeral artery in response to ischemia. the dilatation is evaluated by echotracking. glycocalyx thickness is measured by the study of sublingual microcirculation with SDF imaging. oxydative, inflammatory and coagulation parameters is assessed on plasma samples.

Arms, Groups and Cohorts

  • Experimental: hydroxychlorquine
    • hydroxychloroquine 200 mg twice a day for 6 months
  • Placebo Comparator: control
    • placebo 2 pills a day for 6 months

Clinical Trial Outcome Measures

Primary Measures

  • Change from baseline flow mediated dilatation of brachial artery
    • Time Frame: 6 months
    • The brachial artery diameter and blood flow are measured by echotracking and Doppler before and just after and ischemic test. Result expressed in percentage of diameter variation.

Secondary Measures

  • change from baseline in endothelial glycocalyx thickness
    • Time Frame: 6 months
    • indirect measure of the glycocalyx thickness by using sublingual SDF (sidestream dark field) imaging
  • change from baseline in oxydative stress
    • Time Frame: 6 months
    • plasma levels of nitrites and TBARS (thiobarbituric acid reactive substance)
  • change from baseline in systemic inflammation
    • Time Frame: 6 months
    • plasma levels of TNFalpha
  • change from baseline in coagulation parameter
    • Time Frame: 6 months
    • Tissue factor plasmatic level
  • change from baseline in plasmatic level in hydroxychloroquine
    • Time Frame: 6 months
    • plasma level of hydroxychloroquine

Participating in This Clinical Trial

Inclusion Criteria

  • Patients who fulfilled Sidney criteria for APS (antiphospholipid Syndrome – Women of childbearing potential must have a contraceptive method – Written informed consent – no severe, progressive, or uncontrolled kidney, liver, blood, stomach, lung, heart, or brain disease. Exclusion Criteria:

  • secondary antiphospholipid syndrome – Pregnancy and breastfeeding – Patients with a history of severe depression, psychosis, or suicidal ideation – story of intolerance or contra-indication to hydroxychloroquine, lactose, trinitrin – Prior use of hydroxychloroquine in the last 6 months – Chronic heart failure – atrial fibrillation – severe pulmonary hypertension – severe kidney failure clearance < 30ml/mn – uncontrolled arterial hypertension – secondary arterial hypertension – diabetes mellitus diagnosed in the last 3 months – body mass index > 35 – Patient has been committed to an institution by legal or regulatory order

Gender Eligibility: All

Minimum Age: 18 Years

Maximum Age: N/A

Are Healthy Volunteers Accepted: No

Investigator Details

  • Lead Sponsor
    • University Hospital, Rouen
  • Provider of Information About this Clinical Study
    • Sponsor
  • Overall Official(s)
    • Sébastien MIRANDA, MD, Principal Investigator, Rouen University Hospital
  • Overall Contact(s)
    • Sébastien MIRANDA, MD, sebastien.miranda@chu-rouen.fr

Clinical trials entries are delivered from the US National Institutes of Health and are not reviewed separately by this site. Please see the identifier information above for retrieving further details from the government database.

At TrialBulletin.com, we keep tabs on over 200,000 clinical trials in the US and abroad, using medical data supplied directly by the US National Institutes of Health. Please see the About and Contact page for details.