Measurement of the Free Fraction of 25-hydroxyvitamin D in Serum

Overview

In the circulation 25-hydroxyvitaminD (25(OH)D) is bound to the vitamin D binding protein (DBP) and albumin. According to the free hormone hypothesis, it is, however, the free fraction that is biologically active. Polymorphisms in DBP are related to the serum level of 25(OH)D. As these polymorphisms may also affect the binding affinities for 25(OH)D, the total serum 25(OH)D may not necessary reflect the free fraction. To test this hypothesis, we will calculate the free fraction of 25(OH)D by correction for DBP and albumin content, and also measure free 25(OH)D from equilibrium dialysis and ultra filtration. Furthermore, we will relate total serum 25(OH)D as well as the free and biologically active (free- albumin-bound) 25(OH)D to the well established vitamin D effect marker serum parathyroid hormone as well as to the RNA expression in peripheral blood to evaluate the biological importance of the free versus the total fraction of 25(OH)D. We will invite 300 subjects from an ongoing vitamin D supplementation study to participate in the study which will be one visit only and include collection of blood samples.

Study Type

  • Study Type: Observational
  • Study Design
    • Time Perspective: Cross-Sectional
  • Study Primary Completion Date: June 2015

Arms, Groups and Cohorts

  • Subjects with impaired glucose tolerance

Clinical Trial Outcome Measures

Primary Measures

  • Free fraction of 25(OH)D
    • Time Frame: 1 day

Secondary Measures

  • DBP polymorphisms
    • Time Frame: 1 day

Participating in This Clinical Trial

Inclusion Criteria

  • Impaired glucose tolerance – living in the Tromsø area Exclusion Criteria – pregnancy – serious illness

Gender Eligibility: All

Minimum Age: 25 Years

Maximum Age: 85 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

  • Lead Sponsor
    • University of Tromso
  • Provider of Information About this Clinical Study
    • Sponsor

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