Metabolic Availability of Lysine From Rice

Overview

The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.

Full Title of Study: “Application of the Indicator Amino Acid Oxidation Technique for the Determination of Metabolic Availability of Lysine From Cooked Rice Protein in Adult Men”

Study Type

• Study Type: Interventional
• Study Design
  • Allocation: N/A
  • Intervention Model: Single Group Assignment
  • Primary Purpose: Health Services Research
  • Masking: None (Open Label)
• Study Primary Completion Date: November 2011

Detailed Description

The investigators objective is to determine the metabolic availability (MA) of lysine in cooked white rice protein using the indicator amino acid oxidation (IAAO) technique. Five men will receive graded levels (20, 40, and 60, and 70%) of the lysine requirement of 35 mg/kg/d as a crystalline AA mixture,and cooked rice protein (20, 40 and 60%), respectively.

Interventions

• Dietary Supplement: Amino Acid Crystalline Lysine Amino Acid Mix
  • crystalline lysine from AA MIX (20, 40, and 60% of the mean lysine requirement of 35 mg/kg/d) Lysine will be given in the form of L-Lysine-HCl, Sigma-Aldrich Brand.
• Dietary Supplement: Cooked Rice containing Lysine
  • Lysine (20,40 and 60 % of the mean lysine requirement) from Cooked Rice

Arms, Groups and Cohorts

• Experimental: Lysine Amino Acid

Clinical Trial Outcome Measures

Primary Measures

• Metabolic Availability of lysine
  • Time Frame: Baseline
  • 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.
• Metabolic Availability of lysine
  • Time Frame: 15 min after intake of the fourth hourly meal (before tracer infusion))
  • 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.
• Metabolic Availability of lysine
  • Time Frame: 30 min after intake of the fourth hourly meal (before tracer infusion))
  • 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.
• Metabolic Availability of lysine
  • Time Frame: 45 min after intake of the fourth hourly meal (before tracer infusion))
  • 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Secondary Measures

• Metabolic Availability of Lysine
  • Time Frame: Post Tracer Infusion (between 150 and 240 min of tracer infusion)
  • 4 breath samples will be taken to provide F13CO2 measurement (Level of phenylalanine oxidation).Breath samples will be collected and analyzed for 13CO2 enrichment.

Participating in This Clinical Trial

Inclusion Criteria

• Healthy Adult males (18 to 50years old) Exclusion Criteria:

• A history of recent weight loss or illness – Use of any medication at the time of entry into the study.

Gender Eligibility: Male

Minimum Age: 18 Years

Maximum Age: 50 Years

Are Healthy Volunteers Accepted: Accepts Healthy Volunteers

Investigator Details

• Lead Sponsor
  • The Hospital for Sick Children
• Provider of Information About this Clinical Study
  • Principal Investigator: Dr. Paul Pencharz, Professor of Paediatrics and Nutritional Sciences (Emeritus) University of Toronto, Senior Scientist Research Institute,The Hospital for Sick Children – The Hospital for Sick Children
• Overall Official(s)
  • Paul B Pencharz, MD, PhD, Principal Investigator, The Hospital for Sick Children, Toronto, Canada