Inflammatory Cytokines Profile in Individuals Subjected to Surgical Procedures Using Propofol or Isoflurane

Overview

The aim of this study is to assess the profile of cytokines IL-1beta, IL-6, IL-8, IL-10, IL-12 and TNF-alpha under inhalation anesthesia with isoflurane and intravenous anesthesia with propofol in healthy patients subjected to minimally invasive elective surgeries.

Full Title of Study: “Inflammatory Cytokines Profile in Individuals Subjected to Surgical Procedures Using Propofol or Isoflurane.”

Study Type

  • Study Type: Interventional
  • Study Design
    • Allocation: Randomized
    • Intervention Model: Parallel Assignment
    • Primary Purpose: Supportive Care
    • Masking: Double (Participant, Investigator)
  • Study Primary Completion Date: December 2009

Detailed Description

Twenty ASA-I patients, subjected to otorhinolaryngology surgery, were randomly allocated in one group to receive anesthesia with isoflurane 1 MAC (minimum alveolar concentration) (n = 20). Other group with twenty ASA-I patients received propofol 2 to 4 microgram mL-1 (n = 20). Fentanyl 5 mg kg-1 and rocuronium bromide 0.6 mg kg-1 were also administered to all patients. Venous blood (10 mL) was collected from each patient at each of the following times: before the beginning of surgery and anesthesia (T1), 2 h after the beginning of surgery (T2), and on the day after the anesthetic-surgical procedure (T3). Plasma concentrations of interleukins IL-1beta, IL-6, IL-8, IL-10 and IL-12 and tumor necrosis factor (TNF-alpha) were measured in each sample through flow cytometry technique by using the method Cytometric Bead Array (CBA). Venous blood samples from fifteen volunteers not subjected to stress were also collected as control, and the same cytokines were measured.

Interventions

  • Drug: Propofol and isoflurane
    • Comparison of two anesthetic drugs

Clinical Trial Outcome Measures

Primary Measures

  • Evaluation of cytokines profile
    • Time Frame: Before surgery and anesthesia, 2h after anesthesia and at the following day of the surgery

Secondary Measures

  • Evaluation of pro and anti-inflammatory cytokines
    • Time Frame: before surgery and anesthesia, 2h after anesthesia and at the following day of the surgery

Participating in This Clinical Trial

Inclusion Criteria

  • Patients in good health American Society of Anesthesia status physical I – Elective minor surgery – General anesthesia Exclusion Criteria:

  • Smokers – Alcoholics – Previous medication or radiation

Gender Eligibility: All

Minimum Age: 18 Years

Maximum Age: 50 Years

Are Healthy Volunteers Accepted: No

Investigator Details

  • Lead Sponsor
    • UPECLIN HC FM Botucatu Unesp
  • Collaborator
    • Fundação de Amparo à Pesquisa do Estado de São Paulo
  • Provider of Information About this Clinical Study
    • Upeclin HC FM Botucatu Unesp, São Paulo State University, UNESP
  • Overall Official(s)
    • Marina Á Mazoti, MsC, Principal Investigator, Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP

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